Research Assistant II/III - Center for Stem Cell and Organoid Medicine-148361
Description
Expected Starting Salary Range: 19.73 - 24.05
Center for Stem Cells and Organoids Medicine (CuSTOM) is soliciting applications for a Research Assistant II/III position in CuSTOM Accelerator Lab (CA). This individual will be responsible for deriving and expanding the necessary cell types to develop stem-cell based preclinical tools for drug discovery and development. In addition, this candidate will be in charge of maintaining autologous organoids as a solution for end-stage intestinal disease and other disorders. He/she will support a variety of internal R&D projects and collaborations with industry partners (biotech and pharma) by performing quantitative polymerase chain reaction assays, fluorescence microscopy, flow cytometry and various plate-based assays on daily basis. This position is part of a multidisciplinary team led by experienced stem cell and developmental biologists and engineers. The ideal candidate has previous hands-on experience in mammalian cell culture, various laboratory techniques outlined above, and a good understanding of cell biology. Experience in stem cell derivation, expansion, and differentiation is a plus.
Research Assistant will be responsible for:
- Perform induced pluripotent stem cell (iPSC) culturing, including freeze, thaw, culturing, quality control testing.
- Perform directed differentiation of iPSCs to various tissue lineages, mainly liver and intestinal organoids.
- Perform PCR, RT-PCR, and other common molecular biology techniques.
- Perform flow cytometry and cell-sorting.
- Perform immunohistochemistry and immunofluorescent staining.
- Perform microscopy, including confocal.
- Maintain records of results and methodologies in a log of work performed.
- Maintain equipment, lab area and supplies in a neat professional manner
- Prepare media and other materials using appropriate procedures
- Carry out all laboratory activities in compliance with applicable regulations and protocols
- Placing ordering on laboratory reagents.
- Conduct literature searches and reviews, compiling information for journals and suggesting uses for new techniques
- Attend courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information
Qualifications
EDUCATION/EXPERIENCE
Required for RAII:
- High school diploma or equivalent
- 2 years of work experience in a related job discipline. or equivalent combination of education and experience
- May require an Associate degree, vocational training, apprenticeships or equivalent
Required for RAIII:
- Bachelor's degree in a related field
- 1 year of work experience in a related job discipline OR Master's degree in a related field
Preferred:
- Bachelor’s Degree in cellular biology, developmental biology, biomedical engineering or related field
- Experience in a cell biology, developmental biology or biomedical engineering in academia or industry
- Aseptic Technique and mammalian cell culture
- Hands-on experience in common laboratory techniques such as qPCR, microscopy, ELISA, flow cytometry etc.
- Knowledge of cell biology
- Ability to initiate and manage day-to-day tasks effectively, including multi-tasking, flexibility and adaptability
- Ability to independently follow experimental methods and protocols
- Comfortable working in a hands-on laboratory environment and have good attention to detail and good lab practices
- Strong attention to detail
Cincinnati Children's is proud to be an Equal Opportunity Employer that values and treasures Diversity, Equity, and Inclusion. We are committed to creating an environment of dignity and respect for all our employees, patients, and families (EEO/AA).
Job
Research
Primary Location
United States-Ohio-Cincinnati-Burnet Building Location R
Schedule
Full-time
Shift
Day Job
Job Type
Standard
Department 597000 - CuSTOM Program
Employee Status
Regular
FTE 1
Weekly Hours 40
Salary Range
19.73-28.37
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